CaptionsMaker
.com
Measuring Size, Stability and Conformation of Biopolymers with Dynamic Light Scattering
Edit Subtitles
Download Subtitles
SRT
TXT
Title:
Description:
Light scattering (LS), including classical and dynamic, has been widely employed to characterize protein solutions and other biomolecules. Classical or static light scattering, especially multi-angle light scattering (MALS), determines the absolute molecular weight of macromolecules in solution, facilitating the characterization of molecular heterogeneity and impurity profile. Dynamic light scattering (DLS), also known as quasi-elastic light scattering (QELS), directly measures the translational diffusion coefficient from which the hydrodynamic radius of a molecule is derived. Both static and dynamic light scattering can be used either in stand-alone (unfractionated, batch) mode or on-line coupled with a separation techniques, such as size exclusion chromatography (SEC) or field-flow fractionation (FFF).
YouTube url:
https://www.youtube.com/watch?v=oISKEZ-DHpA
Created:
31. 3. 2022 19:05:57